Definition: An analytical transmission electron microscopy method using an electron microscope fitted with an energy filtering lens. The method is based on the principle that some of the ELECTRONS passing through the specimen will lose energy when they ionize inner shell electrons of the atoms in the specimen. The amount of energy loss is dependent upon the element. Analysis of the energy loss spectrum (ELECTRON ENERGY-LOSS SPECTROSCOPY) reveals the elemental composition of a specimen. It is used analytically and quantitatively to determine which, how much of, and where specific ELEMENTS are in a sample. For example, it is used for elemental mapping of PHOSPHORUS to trace the strands of NUCLEIC ACIDS in nucleoprotein complexes.
Other names TEM, Energy-Filtered; Spectroscopic Imaging, Electron; Microscopy, Energy Filtering Transmission Electron; Imaging, Electron Spectroscopic; Energy Filtering Transmission Electron Microscopy; Energy Filtered Transmission Electron Microscopy; Energy Filtered TEM; Energy-Filtering Transmission Electron Microscopy; Energy-Filtered TEM; Electron Spectroscopic Imaging; EFTEM; EF-TEM; Energy-Filtered Transmission Electron Microscopy
